Input data are a protein sequence and amino acid variations.

Amino acid sequence

Accepted formats are the following;
・Header beginning with “>” followed by 1-16 alphanumeric characters or underscore(”_”)
・No white space allowed
・Amino acids in one-letter code
・More than 30 residues


Accepted formats are the following;

<Reference amino acid><Position><Variant amino acid>   e.g. D15L

・Amino acids in one-letter code
・Position indicates the location number of reference amino acid residue from the first residue in your query sequence as position 1.
・No white space allowed
・Only single site substitutions, no deletions and insertions
・A list of multiple substitutions are separated by comma(,) or linefeed.
・No more than 50 mutations

Prediction tools

Prediction Methods Version Developer/Provider Reference
PANTHER ・Evolutionary conservation 1.02 ・University of Southern California (Thomas laboratory) Website
Thomas et al., 2003
Mi et al., 2012
PolyPhen2 ・Evolutionary conservation
・Machine learning (Bayesian)
2.2.2r405 ・Brigham & Women’s Hospital (Harvard Medical School) Website
Adzhubei et al., 2010
PROVEAN ・Evolutionary conservation 1.1 ・J. Craig Venter Institute Website
Choi et al., 2012
SIFT ・Evolutionary conservation 1.03 ・J. Craig Venter Institute
Kumar et al., 2009


The result URL will be sent by e-mail. Analysis may take quite some time; from several minutes to days. This service is optional, but strongly recommended.

Prediction score

The higher score represents the more neutral mutation except in PolyPhen2.

Min. score Cutoff Max. score
-3 0
PolyPhen2 0
0.5 1
(Probably damaging)
-2.5 -
0.05 1